Genetic Relationship in Strawberry Cultivars and Their Progenies Analyzed by Isozyme and RAPD

Abstract

Isozyme pattern and RAPD technique were used to study the relationship of new hybrids in strawberry breeding in Thailand. The selected progenies from alternate crosses and their parental lines were investigated and the phylogenetic trees were constructed. Some polymorphic relations were observed when the four enzyme systems; LAP, MDH, ME and DIA were used, however they could not clearly distinct hybrid lines uniquely at either 90 or 95% similarity levels. RAPD technique, using 10 random primers, could effectively show most of the hybrid lines uniquely at 95% similarity level. Moreover, RAPD differentiated strawberry cultivars effectively. Data from this study on the hybrid line relationship could be useful for parental selection in strawberry improvement program.