Natural killer T follicular helper (NKTFH) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKTFH cells by multiparameter flow cytometer.
CITATION STYLE
Chang, P. P., & Vinuesa, C. G. (2015). Detection of mouse natural killer T follicular helper (NKTFH) cells by flow cytometry. In T Follicular Helper Cells: Methods and Protocols (pp. 135–141). Springer New York. https://doi.org/10.1007/978-1-4939-2498-1_11
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