Bioelectronic DNA detection of human papillomaviruses using eSensor™: A model system for detection of multiple pathogens

Abstract

Background: We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens. Methods: Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes. Results: Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples. Conclusions: This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens. © 2003 Vernon et al; licensee BioMed Central Ltd.