Keeping eyes peeled: guppies exposed to chemical alarm cue are more responsive to ambiguous visual cues

Abstract

Abstract: Information received from the visual and chemical senses is qualitatively different. For prey species in aquatic environments, visual cues are spatially and temporally reliable but risky as the prey and predator must often be in close proximity. Chemical cues, by contrast, can be distorted by currents or linger and thus provide less reliable spatial and temporal information, but can be detected from a safe distance. Chemical cues are therefore often the first detected and may provide a context in which prey respond to subsequent ambiguous cues (“context hypothesis”). Depending on this context, early chemical cues may also alert prey to attend to imminent cues in other sensory modalities (“alerting hypothesis”). In the context of predation risk, for example, it is intuitive that individuals become more responsive to subsequent ambiguous cues across sensory modalities. Consistent with the context hypothesis, guppies, Poecilia reticulata, exposed to conspecific alarm cue reduced activity, a classic fright response among fish, in response to a water disturbance more than those exposed to cues of unharmed conspecifics or a water control. Despite this reduction in activity, guppies exposed to alarm cue were more attentive to visual cues than those exposed to the other chemical cues, as predicted by the alerting hypothesis. These responses contrasted with those of guppies exposed to chemical cues of undisturbed, unharmed conspecifics, which were relatively unaffected by the disturbance. This is the first study indicating that unambiguous cues detected by one sensory modality affect animal responses to subsequent ambiguous multimodal cues. Significance statement: In moving water, chemical cues can be detected over longer distances than visual cues; they may therefore be detected first and alert animals to imminent visual cues. This effect is likely to be particularly important if these chemical cues are indicative of predation. I investigated how different chemical cues affect (1) guppy response to an ambiguous water disturbance and (2) their responsiveness to subsequent ambiguous visual cues. Guppies based their responses to ambiguous cues on the context implied by chemical cues: those exposed to chemical cues indicative of predation reduced activity, a classic fright response, but increased responsiveness to visual cues, relative to those exposed to control chemical cues. This is the first study to show that unambiguous cues detected by one sense affect animal responses to ambiguous cues detected by other senses.