In order to comprehend the function of a particular protein, identification of the interacting protein partners is a useful approach. Co-immunoprecipitation (Co-IP) is employed to test physical interactions between proteins. Specific antibodies or antibodies against tagged versions can be used to immunoprecipitate the proteins. In this chapter, we describe a method to carry out Co-IP using recombinant membrane proteins expressed in yeast microsomal fractions.
CITATION STYLE
Barberini, M. L., & Muschietti, J. P. (2017). Co-immunoprecipitation of plant receptor kinases. In Methods in Molecular Biology (Vol. 1621, pp. 109–112). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7063-6_10
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