The ISG15 conjugation system

29Citations
Citations of this article
30Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

ISG15 is a ubiquitin-like modifier that is expressed in response to type 1 interferon signaling (IFN-α/β) and plays a role in antiviral responses. The core E1, E2, and E3 enzymes for ISG15 are Ube1L, UbcH8, and Herc5, respectively, and these are all also induced at the transcriptional level by IFN-α/β. We recently showed that Herc5 associates with polysomes and modifies target proteins in a cotranslational manner. Here, we describe the expression of the core conjugating enzymes in human cells, the detection of ISG15 conjugates, and the methods for fractionation of Herc5 with polysomes. © 2012 Springer Science+Business Media, LLC.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Durfee, L. A., & Huibregtse, J. M. (2012). The ISG15 conjugation system. Methods in Molecular Biology, 832, 141–149. https://doi.org/10.1007/978-1-61779-474-2_9

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 15

63%

Researcher 6

25%

Professor / Associate Prof. 3

13%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 10

45%

Biochemistry, Genetics and Molecular Bi... 8

36%

Immunology and Microbiology 3

14%

Medicine and Dentistry 1

5%

Save time finding and organizing research with Mendeley

Sign up for free