Antimutagenic effect of essential oil of sage (Salvia officinalis L.) and its fractions against UV-induced mutations in bacterial and yeast cells

Abstract

The inhibition of spontaneous and UV-induced mutations by essential oil (EO) of sage (Salvia officinalis L.) and its fractions F1-F5 containing different proportions of mono- and sesquiterpenes was studied with the Salmonella/microsome, E. coli K12, and S. cerevisiae D7 reversion assays. The EO, F1, and F2 exhibited antimutagenic potential against UV-induced mutations in all tests. Fractions F3 and F4 produced a toxic, mutagenic, or antimutagenic response, depend?ing on the test organism used. Reduction of spontaneous and UV-induced mutations by F5 was detected only in permeable strains of E. coli. The obtained results demonstrate antimutagenic activity of volatile sage terpenes and recommend them for further antimutagenesis and anticarcinogenesis studies.Inhibicija spontanih i UV-indukovanih mutacija etarskim uljem (EO) zalfije (Salvia officinalis L.) i njegovim frakcijama F1-F5, sa razlicitim udelom mono- i seskviterpena, ispitivana je u Salmonella/mikrozom, E. coli K1 2 i S. cerevisiae D7 reverznim testovima. U svim testovima EO, F1 i F2 su pokazale antimutageni potencijal prema UV-indukovanoj mutagenezi. U zavisnosti od primenjenog test organizma, frakcije F3 i F4 su pokazale toksicni, mutageni ili antimutageni efekat. F5 frakcija je redukovala spontanu i UV-indukovanu mutagenezu samo u propustljivim slojevima E. coli K12. Dobijeni rezultati pokazuju antimutagenu aktivnost isparljivih terpena iz zalfije i preporucuju ih za dalja ispitivanja u oblasti antimutageneze i antikancerogeneze.