The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction

Abstract

Enzyme hydrolysis optimisation of cell-wall polysaccharides of pumpkin seed with celluloses and pectinases and their mixture, in the relation to enhanced protein extraction was the objective of this study. The individual and combined effects of cell-wall-degrading enzyme activities on protein extraction combined with other process parameters: enzyme concentration and time of hydrolysis were evaluated by the Response Surface Methodology (RSM). The optimal value protein isolate yield (9 g of soluble protein from J 00 g pumpkin seed) is achieved under the following conditions: 2% enzymes (celluloses), reaction time 300 min, pH = 5.0, temperature 45?C.Cilj ovog rada je bio ispitivanje uslova enzimske hidrolize polisaharida celijskog zida semena uljane tikve golice, primenom enzima pektinaza i celulaza kao i njihove smese, radi optimizacije ekstrakcije proteina. Ispitano je delovanje pojedinacnih i kombinovanih aktivnosti enzimapektinaze i celulaze na ekstrakciju proteina, kombinovano sa drugim procesnim parametrima: koncentracija enzima i vreme hidrolize, primenom metodologije odzivnih povrsina. Optimalna vrednost prinosa proteinskog izolata (9 g solubilizovanih proteina iz 100 g semena uljane tikve) je postignuta primenom sledecih reakcionih uslova: 2% enzima (celulaze), vreme reakcije 300 min., rN = 5,0 i temperatura 45?S.