A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5′-bromo-deoxyuridine for two rounds of DNA replication followed by collecting metaphase spreads on glass slides, treatment with the UV-sensitive dye Hoechst 33258, long-wave UV light exposure, and Giemsa staining gives a permanent record of the exchanges.
CITATION STYLE
Stults, D. M., Killen, M. W., Marco-Casanova, P., & Pierce, A. J. (2020). The sister chromatid exchange (SCE) assay. In Methods in Molecular Biology (Vol. 2102, pp. 441–457). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0223-2_25
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