Volatile-mediated between-plant communication in Scots pine and the effects of elevated ozone

Abstract

Conifers are dominant tree species in boreal forests, but are susceptible to attack by bark beetles. Upon bark beetle attack, conifers release substantial quantities of volatile organic compounds known as herbivore-induced plant volatiles (HIPVs). Earlier studies of broadleaved plants have shown that HIPVs provide information to neighbouring plants, which may enhance their defences. However, the defence responses of HIPV-receiver plants have not been described for conifers. Here we advance knowledge of plant-plant communication in conifers by documenting a suite of receiver-plant responses to bark-feeding-induced volatiles. Scots pine seedlings exposed to HIPVs were more resistant to subsequent weevil feeding and received less damage. Receiver plants had both induced and primed volatile emissions and their resin ducts had an increased epithelial cell (EC) mean area and an increased number of cells located in the second EC layer. Importantly, HIPV exposure increased stomatal conductance and net photosynthesis rate of receiver plants. Receiver-plant responses were also examined under elevated ozone conditions and found to be significantly altered. However, the final defence outcome was not affected. These findings demonstrate that HIPVs modulate conifer metabolism through responses spanning photosynthesis and chemical defence. The responses are adjusted under ozone stress, but the defence benefits remain intact.