Production and Characterization of Nanoparticles Lipid Carrier (NLCs) Loaded with Red Clover Isoflavones Extract and Their effect on Serum Lipid profile in Postmenopausal Period

Abstract

Plant-derived edible nanoparticles (PDNPs) are nano-sized membrane vesicles released by edible plants. They are non-toxic, have tissue-specific targeting properties, and can be mass-produced , to assess the effect of phytoestrogens (PEs) for treating hyperlipidemia that associated with menopause period using nanoparticles lipid carrier (NLCs) loaded with red clover isoflavones extract (RCIE) compared with hormone replacement therapy (HRT). Study was conducted using fifty adult female mice model for menopause using 4-Vinylcyclohexene dioxide (VCD) and handled as follows for 6 weeks. Two experiments were preformed, the first experiment included 20 mice divided into two groups Group A: Control group has injected 0.1 ml D.W. intraperitoneal (IP) daily. Group B: This group has injected IP daily 160 mg/kg B.W of VCD. The second experiment was included 30 mice have injected IP daily 160 mg/kg of VCD and divided into three groups (C, D and E), (10 / group) then treated with dermal sticker for six weeks: Group C: treated with dermal sticker saturated with 10ug/ kg B.W of estradiol benzoate (EB) diluted with virgin coconut oil (VCNO). Group D:  treated with dermal sticker saturated with 0.1 of VCNO.  Group E: treated with dermal sticker saturated with 0.1ml of RCIE-NLCs. The results of statistical analysis showed a significant increase in the level of TC, TG, LDL, and VLDL in group VCD. While RCIE-NLCs had a clear effect  and significant decreased in the TG, TC, LDL and VLDL cholesterol compared to groups VCD and EB, and significant increase in the HDL-C in group RCIE-NLCs compared to groups VCD and EB. The results of this study revealed that administration of RCIE-NLCs shows an effective to regulation of lipids metabolism disorders due to hormonal changes that associated with menopausal transition via impact of VCD.