This work focuses on the use of a co-product of biodiesel production as carbon and energy source for the production of rhamnolipids by a new strain of Pseudomonas aeruginosa MSIC02. Different carbon sources were compared and the highest rhamnolipid concentration (1269.79 mg/L) was achieved when hydrolyzed glycerin was used. The study of nutritional and environmental conditions allowed an increase in the production of rhamnolipids. Maximum rhamnolipid concentration was attained at 18 g/L of glycerol, 4.0 g/L of NaNO3, 62 mM of KH2PO4, pH 7.0 and 37 °C. Highest product yields on substrate and biomass and productivity were achieved at the same conditions. The biosurfactant showed good emulsifying properties (IE 24 = 65%), being able to form emulsions with mineral and vegetable oils. The NMR spectrum of H1 and C13 and mass spectra indicates that the purified product contained two types of rhamnolipids: l-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (RL1) and l-rhamnosyl l-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (RL2). © 2011 Elsevier Ltd. All rights reserved.
CITATION STYLE
De Sousa, J. R., Da Costa Correia, J. A., De Almeida, J. G. L., Rodrigues, S., Pessoa, O. D. L., Melo, V. M. M., & Gonalves, L. R. B. (2011). Evaluation of a co-product of biodiesel production as carbon source in the production of biosurfactant by P. aeruginosa MSIC02. Process Biochemistry, 46(9), 1831–1839. https://doi.org/10.1016/j.procbio.2011.06.016
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