This work focuses on the use of a co-product of biodiesel production as carbon and energy source for the production of rhamnolipids by a new strain of Pseudomonas aeruginosa MSIC02. Different carbon sources were compared and the highest rhamnolipid concentration (1269.79 mg/L) was achieved when hydrolyzed glycerin was used. The study of nutritional and environmental conditions allowed an increase in the production of rhamnolipids. Maximum rhamnolipid concentration was attained at 18 g/L of glycerol, 4.0 g/L of NaNO3, 62 mM of KH2PO4, pH 7.0 and 37 °C. Highest product yields on substrate and biomass and productivity were achieved at the same conditions. The biosurfactant showed good emulsifying properties (IE 24 = 65%), being able to form emulsions with mineral and vegetable oils. The NMR spectrum of H1 and C13 and mass spectra indicates that the purified product contained two types of rhamnolipids: l-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (RL1) and l-rhamnosyl l-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (RL2). © 2011 Elsevier Ltd. All rights reserved.
De Sousa, J. R., Da Costa Correia, J. A., De Almeida, J. G. L., Rodrigues, S., Pessoa, O. D. L., Melo, V. M. M., & Gonalves, L. R. B. (2011). Evaluation of a co-product of biodiesel production as carbon source in the production of biosurfactant by P. aeruginosa MSIC02. Process Biochemistry, 46(9), 1831–1839. https://doi.org/10.1016/j.procbio.2011.06.016