Antibacterial Activities of Endophytic Bacteria Isolate AKEBG28 from Yellow Root Plant ( Arcangelisia flava (L.) Merr) Against Escherichia coli and Staphylococcus aureus

Abstract

Yellow root plant (Arcangelisia flava (L.) Merr) is one of the plants widely used as a medicinal plant. Yellow root plant stems have secondary metabolites of the Alkaloid groups that have the potential as antibacterial. Antibacterials can be produced by microorganisms such as endophyte bacteria. So far, there is no research has been reported on the antibacterial activities of yellow root plant endophyte bacterial isolates. This study aims to determine the antibacterial activities produced by yellow root plant endophyte bacterial isolates against Escherchia coli and Staphylococcus aureus. Endophyte bacterial isolates were collected from the microbiology laboratory, Biology Department, University of Bengkulu. The isolate was repurified on Nutrient Agar (NA) media for further steps. Isolate is further molecularly identified using 16S rRNA genes. The antagonistic results of antibacterial activities using culture, pellets, supernatants showed that isolate AKEBG28 had antibacterial activities against Escherichia coli and Staphylococcus aureus. Molecular identification based on 16S rRNA genes showed that the AKEBG28 isolate was genetically close with genus Bacillus cerreus Strain MS54.