Oldest known multituberculate stapes suggests an asymmetric bicrural pattern as ancestral for Multituberculata

Abstract

Middle ear ossicles (malleus, incus, stapes) are known for few multituberculate taxa, and three different stapedial morphotypes have been suggested: (i) slender, columelliform and microperforate, (ii) robust and rod-like, and (iii) bicrural. Reinvestigation of Upper Jurassic (Kimmeridgian) mammalian petrosals from the Guimarota coal mine in central Portugal (Western Europe) revealed an asymmetric bicrural stapes (ABS) in the paulchoffatiid Pseudobolodon oreas. The middle ear ossicles displaced inside the osseous vestibule were detected by a µCT analysis. The Kimmeridgian age of the Guimarota stapes exceeds the stapes from the Early Cretaceous (Barremian) of Asia (about 122-124 Ma) by approximately 30 Myr, and is only slightly younger than the stapes of the recently described Oxfordian euharamiyidan Arboroharamiya allinhopsoni. The Guimarota stapes indicates that the stapes of Lambdopsalis, described as columelliform and microperforate (small stapedial foramen), does not represent a general condition formultituberculates. The stapes of Pseudobolodon is bicrural, the anterior crus sits centrally on the oval footplate, and the stapedial head is simple and smaller than the footplate.We hypothesize that the ABS evolved from the symmetric bicrural stapes (SBS) of non-mammaliaform cynodonts. The ABS appears to be the ancestral morphotype of the mammalian SBS, and the mammalian columelliform imperforate stapes.