In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles

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Abstract

Purpose: This study aimed to develop an anti-inflammation system consisting of epigallocatechin- 3-gallate (EGCG) encapsulated in poly(lactide-co-glycolic acid) (PLGA) particles to promote wound healing. Methods: Nano- and microscale PLGA particles were fabricated using a water/oil/water emulsion solvent evaporation method. The optimal particle size was determined based on drug delivery efficiency and biocompatibility. The particles were loaded with EGCG. The anti-inflammatory effects of the particles were evaluated in an in vitro cell-based inflammation model. Results: Nano- and microscale PLGA particles were produced. The microscale particles showed better biocompatibility than the nanoscale particles. In addition, the microscale particles released ~60% of the loaded drug, while the nanoscale particles released ~50%, within 48 hours. Thus, microscale particles were selected as the carriers. The optimal EGCG working concentration was determined based on the effects on cell viability and inflammation. A high EGCG dose (100 µM) resulted in poor cell viability; therefore, a lower dose (#50 µM) was used. Moreover, 50 µM EGCG had a greater anti-inflammatory effect than 10 µM concentration on lipopolysaccharide-induced inflammation. Therefore, 50 µM EGCG was selected as the working dose. EGCG-loaded microparticles inhibited inflammation in human dermal fibroblasts. Interestingly, the inhibitory effects persisted after replacement of the drug-loaded particle suspension solution with fresh medium. Conclusion: The EGCG-loaded microscale particles are biocompatible and exert a sustained anti-inflammatory effect.

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Wu, Y. R., Choi, H. J., Kang, Y. G., Kim, J. K., & Shin, J. W. (2017). In vitro study on anti-inflammatory effects of epigallocatechin-3-gallate-loaded nano- and microscale particles. International Journal of Nanomedicine, 12, 7007–7013. https://doi.org/10.2147/IJN.S146296

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