MiR-153 promotes neural differentiation in the mouse hippocampal HT-22 cell line and increases the expression of neuron-specific enolase

Abstract

MicroRNAs (miRNAs) have been foUnd to play important regulatory roles in certain neurodegenerative diseases. The aim of the present study was to investigate the effect of miRNA-153 (miR-153) on the neural differentiation of HT-22 cells. overexpression of miR-153 induced the differentiation of HT-22 cells, increasing the number of protrusions and branches, reducing the S phase distribution of the cell cycle, and attenuating the cell proliferation rate as determined using the cell counting Kit-8 assay. Furthermore, miR-153 increased the expression of neuron-specific y-enolase (NSE), neuronal nuclei (NeuN), and N-ethylmaleimide-sensitive fusion attachment protein 23 (SNAP23) and SNAP25 at the transcriptional and protein level by P C R and western blot analysis. Moreover, miR-153 caused obvious upregulation of peroxiredoxin 5 (P R X 5), which has been foUnd to protect neural cells from death and apoptosis. miR-153 promoted neural differentiation and protected neural cells by upregulating the neuron markers y-enolase, neuronal nuclei, and the functional proteins SNAP23, SNAP25 and P R X 5 . Therefore, miR-153 may be a potential target for the treatment of certain neurodegenerative diseases.