First molecular evidence of Soybean mosaic virus (SMV) infection in soybean from India

Abstract

The occurrence of Soybean mosaic virus (SMV) in soybean grown in mid-hill condition of Meghalaya, India was confirmed by symptomatology, transmission electron microscopy, reverse transcription-polymerase chain reaction (RT-PCR) and partial characterization of cytoplasmic inclusion protein (CI), nuclear inclusion protein and coat protein (NIb-CP) domains. Sequence analysis showed 99.0 % nucleotide and amino acid identities for both domains with previously reported SMV (isolate Ar13) from Iran. This is the first molecular evidence of SMV infection in soybean from India.