In this review, we describe the BD Rhapsody™ Single-Cell Analysis System, a platform that allows high-throughput capture of nucleic acids from single cells using a simple cartridge workflow and a multitier barcoding system. The resulting captured information can be used to generate various types of next-generation sequencing (NGS) libraries, including whole transcriptome analysis for discovery biology and targeted RNA analysis for high sensitivity transcript detection. The BD Rhapsody system can be used with emerging applications, such as BD™ AbSeq assays, to profile gene expression in both mRNA and protein level to provide ultra-high resolution analysis of single cells.
CITATION STYLE
Shum, E. Y., Walczak, E. M., Chang, C., & Christina Fan, H. (2019). Quantitation of mRNA Transcripts and Proteins Using the BD RhapsodyTM Single-Cell Analysis System. In Advances in Experimental Medicine and Biology (Vol. 1129, pp. 63–79). Springer New York LLC. https://doi.org/10.1007/978-981-13-6037-4_5
Mendeley helps you to discover research relevant for your work.