Skeletal muscle tissue is characterized by a population of quiescent mononucleated myoblasts, localized between the basal lamina and sarcolemma of myofibers, known as satellite cells. Satellite cells play a pivotal role in muscle homeostasis and are the major source of myogenic precursors in mammalian muscle regeneration. This chapter describes protocols for isolation and culturing satellite cells isolated from mouse skeletal muscles. The classical procedure, which will be discussed extensively in this chapter, involves the enzymatic dissociation of skeletal muscles, while the alternative method involves isolation of satellite cells from isolated myofibers in which the satellite cells remain in their in situ position underneath the myofiber basal lamina. In particular, we discuss the technical aspect of satellite cell isolation, the methods necessary to enrich the satellite cell fraction and the culture conditions that optimize proliferation and myotube formation of mouse satellite cells.
CITATION STYLE
Musarò, A., & Carosio, S. (2017). Isolation and culture of satellite cells from mouse skeletal muscle. In Methods in Molecular Biology (Vol. 1553, pp. 155–167). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6756-8_12
Mendeley helps you to discover research relevant for your work.