Osteoclasts are highly specialized multinucleated cells derived from the monocyte/macrophage hematopoietic lineage that are uniquely capable of adhering to bone matrix and resorbing bone. The tartrate-resistant acid phosphatase (TRAP) assay is the most common method to detect osteoclasts population in vitro. Here we described a general protocol of inducing osteoclast differentiation from the murine macrophage cell line, RAW264.7, and identification of osteoclasts with the classical TRAP assay.
CITATION STYLE
Yang, J., Bi, X., & Li, M. (2019). Osteoclast differentiation assay. In Methods in Molecular Biology (Vol. 1882, pp. 143–148). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8879-2_12
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