Study of root para-nodules formation in wheat (Triticum durum) inoculated with Frankia strain CcI3 and treated with 2, 4-dichlorophenoxyacetate (2, 4-D)

Abstract

Frankia strains can induce N 2-fixing root nodules on certain non-leguminous plants. It is known that exogenous application of 2,4-dichlorophenoxyacetate (2,4-D) affects root morphology. In this work, wheat roots were treated with 2,4-D and inoculated with the actinomycete Frankia. Wheat plants grew in a growth chamber with hydroponic medium. Binocular observation revealed that para-nodules were formed when wheat roots were inoculated with Frankia and the root length was enhanced. When the inoculation with Frankia was combined to 2,4-D treatment, the para-nodules formed were bigger and more numerous, while the root length was shortened.