Global and quantitative profiling of polyadenylated RNAs using PAS-seq

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Abstract

mRNA alternative polyadenylation (APA) has been increasingly recognized as a widespread and evolutionarily conserved mechanism for eukaryotic gene regulation. Here we describe a method called poly(A) site sequencing that can not only map RNA polyadenylation sites on a transcriptome level but also provide quantitative information on the relative abundance of polyadenylated RNAs. This method has been successfully used for both global APA analysis and digital gene expression profiling.

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APA

Yao, C., & Shi, Y. (2014). Global and quantitative profiling of polyadenylated RNAs using PAS-seq. In Methods in Molecular Biology (Vol. 1125, pp. 179–185). Humana Press Inc. https://doi.org/10.1007/978-1-62703-971-0_16

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