Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: A preliminary study

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Abstract

Nowadays, enzyme-linked immunosorbent assay (ELISA) based detection of Mycobacterium tuberculosis ( M. tuberculosis) antigen triggered interferon-gamma (IFN-g) secretion by blood T cells displays an improved diagnostic value for M. tuberculosis infection. Applications of fluorescence correlation spectroscopy (FCS) have been explored in various subfields of medicine and molecular biology, including detection of a certain biomarker in liquid instead of ELISA. Here, we present a preliminary study of detecting IFN-g using FCS-based technique. © 2014 Elsevier B.V.

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Shen, C., Knapp, M., Puchinger, M. G., Shahzad, A., Gaubitzer, E., Shen, A. D., & Koehler, G. (2014). Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: A preliminary study. Journal of Immunological Methods, 407, 35–39. https://doi.org/10.1016/j.jim.2014.03.016

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