Budding yeast has been utilized as a model system for studying basic mechanisms of autophagy. The cytoplasm-to-vacuole targeting (Cvt) pathway, which delivers some vacuolar enzymes into the vacuole selectively and constitutively, is one of the most characterized examples of selective autophagy in budding yeast. Here we summarize the methods of X-ray crystallography, NMR, and other biophysical analyses to study the structural basis of the Cvt pathway.
CITATION STYLE
Yamasaki, A., Watanabe, Y., & Noda, N. N. (2019). Structural studies of selective autophagy in yeast. In Methods in Molecular Biology (Vol. 1880, pp. 77–90). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8873-0_4
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