Measuring expression levels of G protein-coupled receptors (GPCRs) is an important step for understanding the distribution, function, and regulation of these receptors. A common approach for detecting proteins from complex biological systems is Western blotting. In this chapter, we describe a general approach to Western blotting protein components of the endocannabinoid system using sodium dodecyl sulfatepolyacrylamide gel electrophoresis and nitrocellulose membranes, with a focus on detecting type 1 cannabinoid (CB1) receptors. When this technique is carefully used, specifically with validation of the primary antibodies, it can provide quantitative information on protein expression levels. Additional information can also be inferred from Western blotting such as potential posttranslational modifications that can be further evaluated by specific analytical techniques.
CITATION STYLE
Wager-Miller, J., & Mackie, K. (2016). Western blotting of the endocannabinoid system. In Methods in Molecular Biology (Vol. 1412, pp. 247–254). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3539-0_25
Mendeley helps you to discover research relevant for your work.