Adsorption of 5′-thiolated DNA on a gold electrode surface as studied by a quartz crystal microbalance and electrochemical measurements

Abstract

The adsorption and desorption processes of 5′-thiolated DNA oligomers onto a gold electrode were investigated using a quartz crystal microbalance (QCM). The morphology of the DNA layer adsorbed on the gold surface was observed and characterized using scanning tunneling microscopy (STM) and electrochemical measurements. The results of QCM experiments showed that the surface coverage of tholated DNA was estimated to be ca. 30%. The STM observation and electrochemical measurements revealed the flexibility of the immobilzied DNA on the gold electrode surface. © 2005 The Japan Society for Analytical Chemistry.