Sample preparation for proteomics analysis: Filter-aided sample preparation (FASP) and single-pot solid-phase sample preparation (SP3)

Abstract

The success of a proteomics experiment critically depends on the correct execution of the sample preparation. Gel-based sample preparation is a well-proven method widely used in qualitative and quantitative proteomics. Recently, sample preparation in a single vessel has gained popularity because of the ease to perform, the possibility for large-scale analysis, and the potential to improve sensitivity. In this chapter I describe two sample preparation protocols, filter-aided sample preparation (FASP) and single-pot solid-phase sample preparation (SP3).