Tissue culture for xylem differentiation with arabidopsis leaves

Abstract

Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3–4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.