Arthrobacter cavernae sp. nov., a novel actinobacterium isolated from sediment of karst cave

Abstract

A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China. The isolate grew optimally on R2A agar at 25 °C, pH 8.0 and with 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3% sequence similarity), Arthrobacter alkaliphilus LC6T (97.7%) and Arthrobacter ramosus CCM1646T (97.1%). Genome sequencing revealed a genome size of 4073119 bp and the genomic DNA G+C content was 66.16mol%. Its DNA–DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2%, respectively. The main fatty acids were anteisoC15:0, anteiso-C17:0 and iso-C16:0 . The major respiratory quinone was MK-9(H2 ). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylinositol and unidentified lipids. Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp. nov. is proposed. The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).