Phosphate solubilizing bacteria isolated from Tuban mangrove soil, Indonesia

Abstract

This research aims to find Indonesian local isolates of Phosphate Solubilizing Bacteria (PSB) originated from Tuban Mangrove Center. The composited soil samples was taken by purposive sampling method. The isolation technique was done by serial dilution on pikovskaya media (10-1-10-8 CFU/g soils ). Bacteria was show clear zones around colonies indicated to solubilize phosphate. The potential bacteria were identified by morphological, physiological and molecular (16SrRNA gene). A total of 19 isolates were shown phosphate solubilizing. One isolate was namely 4a has highest phosphate solubilization index (2.36 ± 0.07mm) with similarity 97.28% belong to Kleibsiella variicola based on 16S rRNA gene analysis.