Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applyin..." /> Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many function..." /> Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many function..." />

Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP

  • Wahyuni D
  • Andriani P
  • Ansori A
  • et al.
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Abstract

<p class="IsiAbstrakIndo"><em>Justicia gendarussa </em>Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (<span>α</span> = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. <span lang="EN-GB">Moreover, t</span>he optimum treatment will be used to produce secondary metabolite and seed s<span lang="EN-GB">y</span>nthetic by cell suspension culture.</p>

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APA

Wahyuni, D. K., Andriani, P., Ansori, A. N. M., & Utami, E. S. W. (2017). Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP. Biosaintifika: Journal of Biology & Biology Education, 9(3), 402. https://doi.org/10.15294/biosaintifika.v9i3.11347

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