Immunohistochemistry is a histological technique that allows detection of one or more proteins of interest within a cell using specific antibody binding, followed by microscopic visualization of a chromogenic substrate catalyzed by peroxidase and/or alkaline phosphatase. Here, we describe a method to localize Chikungunya virus (CHIKV) antigens in formalin-fixed and paraffin-embedded infected mouse brain.
CITATION STYLE
Chiam, C. W., Sam, I. C., Chan, Y. F., Wong, K. T., & Ong, K. C. (2016). Immunohistochemical detection of chikungunya virus antigens in formalin-fixed and paraffin-embedded tissues. In Methods in Molecular Biology (Vol. 1426, pp. 235–240). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3618-2_21
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