5-methylcytosine analysis by RNA-BisSeq

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Abstract

5-Methylcytosine (m5C) is a posttranscriptional RNA modification identified in both stable and highly abundant tRNAs and rRNAs, and in mRNAs. Many known or novel m5C sites have been validated by using advanced high-throughput techniques combined with next-generation sequencing (NGS), especially RNA bisulfite sequencing (RNA-BisSeq). Here we introduce an optimized RNA-BisSeq method by using ACT random hexamers to prime the reverse transcription of bisulfite-treated RNA samples to detect the m5C sites.

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Chen, Y. S., Ma, H. L., Yang, Y., Lai, W. Y., Sun, B. F., & Yang, Y. G. (2019). 5-methylcytosine analysis by RNA-BisSeq. In Methods in Molecular Biology (Vol. 1870, pp. 237–248). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8808-2_18

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