High survival of rabbit morulae after vitrification in an ethylene glycol- based solution by a simple method

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Abstract

Rabbit morulae were exposed to a vitrification solution-modified PBS [PB1] medium containing 40% ethylene glycol + 18% Ficoll + 0.3 M sucrose (EFS) for 2, 5, or 10 min at 20°C and were vitrified in liquid nitrogen. When morulae were rapidly warmed, 96% had an intact zona pellucida. When embryos were cultured after removal of the mucin coat, high proportions of them formed blastocoel (79-100%), but the percentage of embryos developed to fully expanded blastocysts decreased with increased exposure time 87%, 40%, and 17%). The survival rate of morulae vitrified after removal of the mucin coat was lower than that of mucin-intact embryos. To assess the development potential in vivo, 131 embryos were vitrified after 2 min of exposure to EFS solution; all the embryos were recovered and 120 were transferred to recipients without removal of the mucin coat, resulting in 78 (65%) full-term fetuses or young. This simple method, which yields high survival both in vitro and in vivo, will be of practical use for vitrifying rabbit embryos.

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Kasai, M., Hamaguchi, Y., Zhu, S. E., Miyake, T., Sakurai, T., & Machida, T. (1992). High survival of rabbit morulae after vitrification in an ethylene glycol- based solution by a simple method. Biology of Reproduction, 46(6), 1042–1046. https://doi.org/10.1095/biolreprod46.6.1042

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