Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.
CITATION STYLE
Tian, Y., Cai, J., Allman, E. L., Smith, P. B., & Patterson, A. D. (2021). Quantitative analysis of bile acid with UHPLC-MS/MS. In Methods in Molecular Biology (Vol. 2194, pp. 291–300). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0849-4_15
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