Kinetics of re-establishing H3K79 methylation marks in global human chromatin

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Abstract

We employ a stable isotope strategy wherein both histones and their methylations are labeled in synchronized human cells. This allows us to differentiate between old and new methylations on pre-existing versus newly synthesized histones. The strategy is implemented on K79 methylation in an isoform-specific manner for histones H3.1, H3.2, and H3.3. Although levels of H3.3K79 monomethylation are higher than that of H3.2/H3.1, the rate of establishing the K79 methylation is the same for all three isoforms. Surprisingly, we find that pre-existing "old" histones continue to be K79-monomethylated and -dimethylated at a rate equal to the newly synthesized histones. These observations imply that some degree of positional "scrambling" of K79 methylation occurs through the cell cycle. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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Sweet, S. M. M., Li, M., Thomas, P. M., Durbin, K. R., & Kelleher, N. L. (2010). Kinetics of re-establishing H3K79 methylation marks in global human chromatin. Journal of Biological Chemistry, 285(43), 32778–32786. https://doi.org/10.1074/jbc.M110.145094

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