The ethylating agent ethyl methanesulfonate (EMS) is widely used for inducing random point mutations. In Arabidopsis, treatment with EMS causes GC-to-AT transitions with great efficiency: it has been estimated that a population of 50,000 well-mutagenized plants harbors one or more transitions in almost every GC pair of the genome. These properties, combined with ease of use, make EMS a mutagen of choice for genetic screens. Here, we describe a protocol for mutagenizing Arabidopsis seed with EMS. In addition, we briefly consider the germ line sectors typically induced by this treatment, and approaches for estimating the rate of induced mutations.
CITATION STYLE
Gillmor, C. S., & Lukowitz, W. (2020). EMS mutagenesis of Arabidopsis seeds. In Methods in Molecular Biology (Vol. 2122, pp. 15–23). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0342-0_2
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