In this study, the biosurfactant production by an Aureobasidium thailandense LB01 was reported for the first time. Different agro-industrial by-products (corn steep liquor, sugarcane molasses, and olive oil mill wastewater) were evaluated as alternative low-cost substrates. The composition of the culture medium was optimized through response surface methodology. The highest biosurfactant production (139 ± 16 mg/L) was achieved using a culture medium containing yeast extract (2 g/L); olive oil mill wastewater (1.5%, w/w); glucose (6 g/L) and KH2PO4 (1 g/L) after 48 h of fermentation. The partially purified biosurfactant exhibited a critical micelle concentration of 550 mg/L, reducing the surface tension of water up to 31.2 mN/m. Its molecular structure was found to be similar to a lauric acid ester. The biosurfactant exhibited a better performance than the chemical surfactant sodium dodecyl sulfate (SDS) in oil dispersion assays, thus suggesting its potential application in bioremediation.
Meneses, D. P., Gudiña, E. J., Fernandes, F., Gonçalves, L. R. B., Rodrigues, L. R., & Rodrigues, S. (2017). The yeast-like fungus Aureobasidium thailandense LB01 produces a new biosurfactant using olive oil mill wastewater as an inducer. Microbiological Research, 204, 40–47. https://doi.org/10.1016/j.micres.2017.07.004