Objective To evaluate the sensitivity and specificity of the quantitative real-time polymerase chain reaction (qPCR) for 16S rDNA gene screening using sonicated fluid from orthopedic implants. Methods A retrospective study was conducted on 73 sonicated fluids obtained from patients with infection associated with orthopedic implants. The samples were subjected to conventional culture and molecular testing using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and qPCR for 16S rDNA. The cycle threshold values were used to define a cut-off of the qPCR of the 16S rDNA for negative and positive cultures.
CITATION STYLE
Ribeiro, V. S. T., Cieslinski, J., Bertol, J., Schumacher, A. L., Telles, J. P., & Tuon, F. F. (2022). Detection of Microorganisms in Clinical Sonicated Orthopedic Devices Using Conventional Culture and qPCR. Revista Brasileira de Ortopedia, 57(4), 689–696. https://doi.org/10.1055/s-0041-1732386
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