Role of muscle progenitor cells in maintaining morphological characteristics of rat soleus muscle during gravitational unloading by means of passive stretch

Abstract

Working hypertrophy of skeletal muscle is usually coupled with activation of satellite cells with subsequent incorporation of their nuclei into muscle fibers. Earlier, it has been repeatedly shown that muscle stretching prevents the development of atrophic alterations and is accompanied by an intensification of protein synthesis. We suggested that the elimination of the proliferative abilities of progenitor cells by γ-irradiation would lead to a partial loss of the ability of muscle fibers to maintain their size. To evaluate the role of progenitor cells in the development of the preventive effect of passive stretching, an experiment was carried out with the 2500 rad local irradiation of a rat shin and subsequent hind-limb suspension or hind-limb suspension with stretch. Passive stretching during hind-limb suspension completely prevented atrophy, the transformation of fibers, and a decrease in the myonuclear number observed in the hind-limb-suspension group. Irradiation produced no action of the preventive effect of passive stretch. The conclusion is made that passive stretch preventive action is also realized in the absence of proliferating satellite cells. © MAIK Nauka 2008.