ISOLATION, EXTRACTION, ANTIOXIDANT ACTIVITY AND STANDARDIZATION OF NATURAL DYES FROM BIXA ORELLANA L. SEEDS AND THE LEAVES OF LAWSONIA INERMIS L.

Abstract

The antioxidant potential of different fractions of ethanol extracts of seeds of Bixa orellana L. (Bixaceae) and ethanolic extracts of leaves of Lawsonia inermis L. were investigated via 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity using ascorbic acid (Vitamin C) as a standard reference. The free radical scavenging activity of annatto extract ranged from 14.40% to 77.95% in Bixa orellana and 16.50% to 71.8 % in Lawsonia inermis relative to ascorbic acid (15% to 86%) at respective concentration between 10µm/ml and 50µm/ml. phytochemical analysis shows the presence of secondary metabolites such as alkaloids, saponin, tannin, terpenoids and phenolic groups. Hence, both the samples have potential source of new natural antioxidant. Another aim of this study is that the people to get the natural dyes instead of synthetic dye. Natural dyes are not only eco-friendly but also protect human skin. These two samples have both dyeing property and medicinal properties.