Apoptosis induction of cervical carcinoma HeLa cells line by dichloromethane fraction of the rinds of garcinia cowa roxb

Abstract

Objective: This study aims to determine the apoptosis induction of HeLa cervical carcinoma cells death by dichloromethane fraction of the rinds of Garcinia cowa Roxb. Methods: Apoptosis induction of HeLa cell line was observed using a double staining method. Results: The result of double staining observation showed that an apoptosis occurs which marked with yellowish green fluorescence and cell fragmentation. The average percentage of apoptotic cells was higher in the treated variables (70.38%) compared to the control variables (12.26%). Statistical analysis by Independent Sample T-Test showed apoptosis Sig. (2-tailed) = 0.000 (<0.025). Conclusion: The dichloromethane fraction of G. cowa rind induces apoptosis in HeLa cervical cancer cells.