Co-transplantation of hepatocytes and hepatic stellate cells has been shown to increase the engraftment of transplanted hepatocytes in the liver. Here, we describe a method for the simultaneous isolation of human primary hepatocytes and hepatic stellate cells from the same donor for co-transplantation or for use in in vitro cell culture models.
CITATION STYLE
Lee, S. M. L., Schiergens, T. S., Demmel, M., Thasler, R. M. K., & Thasler, W. E. (2017). Isolation of hepatocytes and stellate cells from a single piece of human liver. In Methods in Molecular Biology (Vol. 1506, pp. 247–258). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6506-9_17
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