Production of genetically modified porcine blastocysts by somatic cell nuclear transfer: Preliminary results toward production of xenograft- competent miniature pigs

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Abstract

Galα1-3Gal (α-Gal epitope) is the major xenoantigenic epitope responsible for hyperacute rejection upon pig-to-human xenotransplantation. Endo-β-galactosidase C (EndoGalC) from Clostridium perfringens can digest the α-Gal epitope. In this study, gene-engineered primary cultured porcine embryonic fibroblasts (PEF) expressing EndoGalC were obtained and subjected to somatic cell nuclear transfer (SCNT) to test whether xenograft-competent pigs can be created. The EndoGalC-expressing PEF clones exhibited highly reduced expression of α-Gal epitope, as revealed by cytochemical staining with BS-I-B4 isolectin, a lectin that specifically binds to α-Gal epitope, and FACS analysis. The pattern of low level of α-Gal epitope expression continued for at least 6 months (more than 10 generations) after isolation. SCNT of nuclei from these cells resulted in the generation of blastocysts that displayed nearly complete loss of α-Gal epitope from their cell surface. This is the first study to demonstrate that SCNT using EndoGalC-expressing PEFs as donors would be useful for production of genetically modified cloned pigs suitable for xenotransplantation. © 2010 by the Society for Reproduction and Development.

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Himaki, T., Watanabe, S., Chi, H., Yoshida, M., Miyoshi, K., & Sato, M. (2010). Production of genetically modified porcine blastocysts by somatic cell nuclear transfer: Preliminary results toward production of xenograft- competent miniature pigs. Journal of Reproduction and Development, 56(6), 630–638. https://doi.org/10.1262/jrd.09-227A

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