The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.
CITATION STYLE
Haas, T. M., Qiu, D., Häner, M., Angebauer, L., Ripp, A., Singh, J., … Jessen, H. J. (2020). Four phosphates at one blow: Access to pentaphosphorylated magic spot nucleotides and their analysis by capillary electrophoresis. Journal of Organic Chemistry, 85(22), 14496–14506. https://doi.org/10.1021/acs.joc.0c00841
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