Toxicity Test LC50 of Pineung Nyen Teusalee Seeds (Areca catechu) Extract by Brine Shrimp Lethality Test (BSLT) Methode

Abstract

Utility of natural ingredients as traditional medicines is growing with increasing in exploration of the types of natural ingredients that can be used as traditional medicines to prevent and treat a disease. However, the use of inappropriate levels can cause a risk of health problems. One of the ingredients used to treat diabetes is young areca nut which is known by the people of Aceh as Pineung Nyen. Pineung Nyen, which is consumed by the community, is not only in the raw form but also has experienced a process of smoking or disalee. Excessive consumption of Pineung Nyen can cause symptoms of poisoning. For this reason, an analysis is needed to determine the safe limits of concentration that can be consumed without causing side effects. Toxicity tests can be carried out in vivo by using the Brine Shrimp Lethality Test (BSLT) method which uses Artemia salina Leach shrimp larvae as test animals. From the phytochemical test results, it was found that Pineung Nyen Teusalee methanol extract contained flavonoids, tannins and saponins. In addition, the methanol extract of Pineung Nyen Teusalee is toxic with an LC50 value of 347.86 mg / L.