In-solution digestion of glycoproteins for glycopeptide-based mass analysis

Abstract

Glycopeptides are generated from the enzymatic digestion of glycoproteins with a specific or nonspecific protease. Whether this enzymatic conversion of glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental work fiow. This chapter outlines an optimized in-solution digestion protocol to prepare samples for glycopeptide-based mass analysis. © Springer Science+Business Media, LLC 2013.