Human HSP70 and modified HPV16 E7 fusion DNA vaccine induces enhanced specific CD8+ T cell responses and anti-tumor effects

Abstract

Cervical cancer is one of the most common cancers in women worldwide and persistent infection with human papilloma virus (HPV)s is considered to be the major risk factor. Millions of women are currently infected with high risk genotypes. Therefore, it is imperative to develop therapeutic vaccines to eliminate established infection or HPV-related disease. In the current study, we generated two potential therapeutic HPV DNA vaccines, SigmE7/MtHSP70 and SigmE7/HuHSP70, using human and mycobacterium tuberculosis HSP70 linked, respectively, to HPV16 mE7 and the signal peptide gene of human CD33. Our comparative evaluation of these two vaccines found that vaccination with the SigmE7/HuHSP70 DNA vaccine induced a stronger E7-specific CD8+ T cell immune response and resulted in a more significant therapeutic effect against E7 expressing tumor cells. Our study may serve as an important foundation and significant reference for future clinical applications.