Biodiversity of Trichoderma antagonist saprophytic fungi and its use for biocontrol of Fusarium wilt disease on shallots at Lombok Island, West Nusa Tenggara, Indonesia

Abstract

One of the obstacles in the development of shallots in West Nusa Tenggara (NTB) is the presence of Fusarium wilt disease. The use of antagonistic saprophytic fungi Trichoderma spp. is a biological control technique. The aim of this research is to explore the biodiversity of Trichoderma spp. and its use for controlling Fusarium wilt disease. Research using exploratory methods conducted at the shallot planting center on Lombok Island included isolation of antagonistic saprophytic fungi, purification and identification of isolates. Furthermore, research was carried out in the laboratory using experimental methods including in-vitro antagonism tests by direct opposition and culture steam. The research was continued in Greenhouses in planta and in field conditions in the Highlands of Sembalun Village, Medium Plains of Santong Village and Senteluk Village Lowlands. The results concluded that: there were 6 isolates of saprophytic fungi that were antagonistic, namely T. viride, T. longibrachiatum, T. koningii, T. piluliferum, T. harzianum, and T. hamatum. The mechanism of antagonism is physically through space competition, mycoparasites and antibiosis. Three isolates, namely T. virede, T. harzianum and T. hamatum, were effective in suppressing Fusarium wilt disease and even causing immunity.