This chapter describes how to collect Michaelis-Menten kinetic data on an enzyme-catalyzed reaction with the isothermal titration calorimetry (ITC) and the single-injection method. ITC measures the heat rate which is directly proportional to the reaction rate. The enthalpy change (ΔrH), Km, kcat, and vmax are determined in a single assay that does not require labeling or immobilization.
CITATION STYLE
Quinn, C. F., & Hansen, L. D. (2019). Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry. In Methods in Molecular Biology (Vol. 1964, pp. 241–249). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9179-2_17
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